PathwayStep4694 PathwayStep4695 PathwayStep4690 PathwayStep4687 PathwayStep4685 PathwayStep4686 PathwayStep4683 PathwayStep4684 PathwayStep4502 Association of Cyclin A:Cdk2 with Cdh1 Dissociation of phospho-Cdh1 from the APC/C complex Phosphorylation of Cdh1 by Cyclin A:Cdk2 Association of Emi1 with Cdc20 Association of Emi1 with Cdh1 Phosphorylation of Cdh1 by Cyclin B1:Cdc2 Regulation of APC/C activators between G1/S and early anaphase GENE ONTOLOGYGO:0051439 Pubmed10465783 Pubmed12049731 Pubmed12208841 Pubmed15678131 Pubmed16508672 Reactome Database ID Release 43176408 Reactome, http://www.reactome.org ReactomeREACT_6837 Reviewed: Peters, JM, 2006-03-27 22:55:09 The APC/C is activated by either Cdc20 or Cdh1. While both activators associate with the APC/C, they do so at different points in the cell cycle and their binding is regulated differently (see Zachariae and Nasmyth, 1999). Cdc20, whose protein levels increase as cells enter into mitosis and decrease upon mitotic exit, only associates with the APC/C during M phase. Cdh1 associates with the APC/C in G1. This interaction is inhibited at other times by Cdk1 phosphorylation. Phosphorylation of Emi1 SCF-beta-TrCP mediated degradation of Emi1 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components