PathwayStep4691 PathwayStep4692 PathwayStep4693 Ubiquitination of Emi1 by SCF-beta-TrCP Phosphorylated Emi1 binds the beta-TrCP in the SCF complex SCF-mediated degradation of Emi1 SCF-beta-TrCP mediated degradation of Emi1 Authored: Lorca, T, Castro, A, 2006-01-26 00:00:00 Edited: Matthews, L, 2006-01-30 00:00:00 Emi1 destruction in early mitosis requires the SCFβTrCP ubiquitin ligase complex. Binding of βTrCP to Emi1 occurs in late prophase and requires phosphorylation at the DSGxxS consensus motif as well as Cdk mediated phosphorylation. A two-step mechanism has been proposed in which the phosphorylation of Emi1 by Cdc2 occurs after the G2-M transition followed soon after by binding of βTrCP to the DSGxxS phosphorylation sites. Emi1 is then poly-ubiquitinated and degraded by the 26S proteasome. GENE ONTOLOGYGO:0051437 Pubmed12791267 Reactome Database ID Release 43174113 Reactome, http://www.reactome.org ReactomeREACT_6821 Reviewed: Peters, JM, 2006-03-27 22:55:09