PathwayStep4723 PathwayStep4726 PathwayStep4725 PathwayStep4724 Mis18 Complex Binds the Centromere. HJURP:CENPA Complex Localizes to the Centromere. Deposition of New CENPA-containing Nucleosomes at the Centromere. RSF Complex Binds the Centromere. Deposition of New CENPA-containing Nucleosomes at the Centromere Authored: May, B, 2010-04-15 Edited: May, B, 2010-04-15 Eukaryotic centromeres are marked by a unique form of histone H3, designated CENPA in humans. In human cells newly synthesized CENPA is deposited in nucleosomes at the centromere during late telophase/early G1 phase of the cell cycle. Once deposited, nucleosomes containing CENPA remain stably associated with the centromere and are partitioned equally to daughter centromeres during S phase. A current model proposes that pre-existing CENPA at the centromere drives recruitment of new CENPA, however this has not been proved.<br>The deposition process requires at least 3 complexes: the Mis18 complex, HJURP complex, and the RSF complex. HJURP binds newly synthesized CENPA-H4 tetramers before deposition and brings them to the centromere for deposition in new CENPA-containing nucleosomes. The exact mechanism of deposition remains unknown. GENE ONTOLOGYGO:0034080 Pubmed17339380 Pubmed19410539 Pubmed19410544 Pubmed19410545 Pubmed19590885 Pubmed19629040 Pubmed19660450 Pubmed20080577 Reactome Database ID Release 43606279 Reactome, http://www.reactome.org ReactomeREACT_22186 Reviewed: Almouzni-Pettinotti, G, Dunleavy, EM, 2010-05-30 Reviewed: Foltz, DR, 2010-06-14