PathwayStep4748 PathwayStep4749 PathwayStep4747 PathwayStep4750 Removal of remaining Flap from the C-strand Recruitment of Dna2 endonuclease to the C strand Removal of RNA primer and dissociation of RPA and Dna2 from the C-strand RPA binds to the Flap on the C-strand Removal of the Flap Intermediate from the C-strand Authored: Blackburn, EH, Seidel, J, 2006-03-09 19:41:08 GENE ONTOLOGYGO:0000722 Pubmed1671046 Reactome Database ID Release 43174437 Reactome, http://www.reactome.org ReactomeREACT_7999 Reviewed: Price, C, 2006-07-13 18:33:38 Two endonucleases, Dna2 and flap endonuclease 1 (FEN-1), are responsible for resolving the nascent flap structure (Tsurimoto and Stillman 1991). The Dna2 endonuclease/helicase in yeast is a monomer of approximately 172 kDa. Human FEN-1 is a single polypeptide of approximately 42 kDa. Replication Protein A regulates the switching of endonucleases during the removal of the displaced flap.