Phosphorylation of TBSMs in LAT Authored: Rudd, C.E., de Bono, B, Garapati, P V, 2008-01-24 15:53:10 EC Number: 2.7.10 Pubmed10811803 Pubmed11752630 Pubmed14510693 Reactome Database ID Release 43202245 Reactome, http://www.reactome.org ReactomeREACT_12421 Reviewed: Trowsdale, J, 2008-02-26 12:02:59 The adaptor molecule LAT (Linker for the Activation of T cells) is a membrane protein that links the TCR signal to the cell activation. It has a total 10 (Y36, Y45, Y64, Y110, Y156, Y161, Y200, Y220, and Y255) conserved TBSMs (tyrosine based signaling motifs) in its cytoplasmic region. These tyrosine residues are phosphorylated by the activated ZAP-70 upon TCR/CD3 complex engagement. Phosphorylation of LAT creates binding sites for the Src homology 2 (SH2) domains of other proteins, including PLC-gamma1, Grb2 and Gads, and indirectly binds SOS, Vav, SLP-76, and Itk. The residues Y200, Y220 and Y255 are responsible for Grb2 binding, Y200 and Y220 but not Y255, are necessary for Gads binding and Y161 for the PLC-gamma1 binding (numbering based on Uniprot isoform 1). has a Stoichiometric coefficient of 5