PathwayStep4501 PathwayStep4498 Inactivation of APC/C via CDC20 sequestration Inactivation of APC/C via direct inhibition of the APC/C complex Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components Authored: Yen, T, 2004-05-05 00:00:00 GENE ONTOLOGYGO:0051436 Pubmed14593737 Reactome Database ID Release 43141405 Reactome, http://www.reactome.org ReactomeREACT_1041 Reviewed: Peters, JM, 2006-03-27 22:55:09 The target of the mitotic checkpoint is the Anaphase Promoting Complex/Cyclosome (APC/C) an E3 ubiquitin ligase that targets proteins whose destruction is essential for mitotic exit. Currently, there are two proposed mechanism by which inhibition of the APC/C is achieved. These mechanisms differ depending on the mechanism of signal transduction. The APC/C may be inhibited directly by association with the Mitotic Checkpoint Complex (MCC) or through the sequestration of its activator, Cdc20.