Wee1-mediated phosphorylation of Cyclin B1:phospho-Cdc2 complexes Phosphorylation of Wee1 kinase by Chk1 Retention of phospho-Cdc25C:14-3-3 complexes within the cytoplasm Association of phospho-Cdc25C(Ser 216) with 14-3-3 proteins Phosphorylation of Cdc25C at Ser216 PathwayStep4478 PathwayStep4475 PathwayStep4474 PathwayStep4477 PathwayStep4476 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex Authored: Matthews, L, 2003-08-05 01:10:00 DNA damage induced activation of the checkpoint kinases Chk1/Chk2(Cds1) results in the conversion and/or maintenance of CyclinB:Cdc2 complex in its Tyrosine 15 phosphorylated (inactive) state. Cdc2 activity is regulated by a balance between the phosphorylation and dephosphorylation by the Wee1/Myt1 kinase and Cdc25 phosphatase. Inactivation of the Cyclin B:Cdc2 complex likely involves both inactivation of Cdc25 and/or stimulation of Wee1/Myt1 kinase activity. Reactome Database ID Release 4375035 Reactome, http://www.reactome.org ReactomeREACT_407