Digestion of triacylglycerols by extracellular CEL (bile salt-dependent lipase) Authored: D'Eustachio, P, 2007-02-02 21:43:49 CEL (bile salt-dependent lipase) catalyzes the hydrolysis of extracellular monoacylglycerols to yield glycerol and a long-chain fatty acid. This reaction, in the lumen of the small intestine, is essential for the complete digestion of milk-derived triacylglycerols in the nursing infant (Bernback et al. 1990). Its importance in adult fat digestion is unclear.<p>While alternative splicing gives rise to two CEL isoforms, only the longer one encodes all of the residues that form the active site of the enzyme (Reue et al. 1991). In vitro, monomeric CEL protein is active even in the absence of bile salts. its activity is greatly increased when it is complexed with two molecules of cholate, chenodeoxycholate, or their glycine or taurine conjugates (Lombardo and Guy 1980), and the predominant form of the enzyme active on lipid micelles in the gut is a dimer of two such complexes (Aubert-Jousset et al. 2004).<p>CEL is synthesized in pancreatic acinar cells and released into the small intestine. It is also synthesized in the mammary gland and is a constituent of breast milk (Lombardo 2001; Bernback et al. 1990). EC Number: 3.1.1.23 Pubmed11514232 Pubmed15265857 Pubmed2066663 Pubmed2318975 Pubmed7350912 Pubmed7350913 Reactome Database ID Release 43192430 Reactome, http://www.reactome.org ReactomeREACT_9412