Exchange of cytosolic citrate for mitochondrial phosphoenolpyruvate Authored: D'Eustachio, P, 2008-09-12 21:47:19 Edited: D'Eustachio, P, 2008-09-12 21:47:19 Pubmed11945784 Pubmed11946196 Pubmed4719206 Pubmed8666394 Pubmed9254007 Reactome Database ID Release 43372449 Reactome, http://www.reactome.org ReactomeREACT_14780 Reviewed: Harris, RA, 2008-09-10 18:47:12 SLC25A1, in the inner mitochondrial membrane, mediates the exchange of cytosolic citrate for mitochondrial phosphoenolpyruvate. The exchange is physiologically irreversible because of the potential of the inner mitochondrial membrane. The gene encoding human SLC25A1 has been cloned and its expression pattern has been characterized (Heisterkamp et al. 1995; Iacobazzi et al. 1997), but the biochemical details of the transport process are inferred from those worked out for the well-characterized rat system (Robinson 1971; Soling et al. 1971; Kleineke et al. 1973). phosphoenolpyruvate [mitochondrial matrix] + citrate [cytosol] => phosphoenolpyruvate [cytosol] + citrate [mitochondrial matrix]