PFKFB1 dimer pPF2K-Pase complex Phosphorylation of PF2K-Pase by PKA catalytic subunit Activated PKA (protein kinase A) phosphorylates serine 36 of the bifunctional 6-Phosphofructo-2-kinase /Fructose-2,6-bisphosphatase (PFKFB1) enzyme. This phosphorylation inhibits the enzyme's phosphofructokinase (PFK-2) activity while activating its phosphatase activity. As a result, cytosolic levels of Fructose-2,6-bisphosphate (F-2,6-P2) are reduced. F-2,6-P2 in turn is a key positive regulator of the committed step of glycolysis, so the net effect of this phosphorylation event is a reduced rate of glycolysis. Authored: Gopinathrao, G, 2005-05-11 18:49:22 EC Number: 2.7.11.11 Pubmed6281764 Reactome Database ID Release 43163773 Reactome, http://www.reactome.org ReactomeREACT_2128 has a Stoichiometric coefficient of 2