SERPINE1 gene WWTR1:SMAD stimulates transcription of SERPINE1 while TGIF:SMAD inhibits it Authored: Orlic-Milacic, M, 2012-04-04 Edited: Jassal, B, 2012-04-10 Knocking down WWTR1 (TAZ) expression by siRNA treatment inhibits TGF-beta-dependent transcription of SERPINE1 (PAI-1 i.e. plasminogen activator inhibitor 1) in hepatocellular carcinoma cell line Hep G2. Chromatin immunoprecipitation (ChIP) confirmed binding of both WWTR1 and SMAD2/3 to the promoter of SERPINE1 gene in response to TGF-beta stimulation (Varelas et al. 2008). Binding of TGIF1 or TGIF2 to SMAD2/3:SMAD4 heterotrimer negatively regulates transcription of SERPINE1 (Wotton et al. 1999, Melhuish et al. 2001). Pubmed18568018 Reactome Database ID Release 432106586 Reactome, http://www.reactome.org ReactomeREACT_120967 Reviewed: Huang, Tao, 2012-05-14