Ligated exon containing complex intron-containing complex Exon Junction Complex RNA polymerase II (phosphorylated):TFIIF complex Cleavage at the 3'-Splice Site and Exon Ligation Reactome Database ID Release 4372160 Reactome, http://www.reactome.org ReactomeREACT_1331 The second step of the splicing reaction results in cleavage of the transcript at the 3'splice site, and results in ligation of the two exons and excision of the intron.