p(S27)-G protein alpha (z):GTP Gz is a substrate for PKC Authored: Jupe, S, 2010-05-18 EC Number: 2.7.11.13 Edited: Jupe, S, 2010-05-24 G alpha z (Lounsbury et al. 1991) and G alpha 12 (Kozasa & Gilman, 1996) are excellent in vitro substrates for all three subtypes of protein kinase C (PKC). Activation of PKC in intact platelets by agents such as thrombin, thromboxane A2 (TXA2) analogues and phorbol esters leads to rapid and near-stoichiometric phosphorylation of G alpha z (Carlson et al. 1989). The primary site of G alpha z phosphorylation is Ser-27 (Lounsbury et al. 1993). This phosphorylation blocks the interaction of G alpha z with Gbeta:gamma suggesting that it is a regulatory mechanism for attenuating signalling by preventing subunit reassociation. Pubmed1939224 Pubmed2502548 Pubmed8429024 Pubmed8647866 Reactome Database ID Release 43751040 Reactome, http://www.reactome.org ReactomeREACT_22249 Reviewed: D'Eustachio, P, 2010-05-21 G-protein alpha (z):GTP