Synthesis of PIP3 from PIP2 by PI3K Authored: Garapati, P V, 2011-07-11 Edited: Garapati, P V, 2011-07-11 PI3 kinases catalyze the production of phosphatidylinositol-3, 4, 5-triphosphate (PIP3) by phosphorylating phosphatidylinositol-4, 5-bisphosphate (PIP2). PIP3 bound to the inner lipid bilayer of the plasma membrane promotes the recruitment and activation of AKT. Active AKT subsequently phosphorylates the pro-apoptotic factor Bad at Ser136 which leads to it binding 14-3-3 protein and sequestering from the anti-apoptotic molecules Bcl-XL therby reducing antiapoptotic events and promoting cell survival. The alternative route for c-Kit mediated survival is through AKT-mediated phosphorylation and incativation of the forkhead transcription factor (FoxO3a) (Engstrom et al, 2003; Lennartson et al , 2005). Pubmed11395417 Pubmed12660731 Pubmed12691919 Pubmed15526160 Pubmed16483568 Pubmed9651683 Reactome Database ID Release 431433514 Reactome, http://www.reactome.org ReactomeREACT_111081 Reviewed: Rönnstrand, L, 2011-08-22