Extracellular matrix ligands PDGF A and B chains with retention motif PDGF A and B chains:ECM complex PDGF binds to extracellular matrix proteins Authored: Garapati, P V, Jassal, B, 2008-11-24 10:14:27 Edited: Garapati, P V, Jassal, B, 2008-11-24 10:14:27 Pubmed10508235 Pubmed1311092 Pubmed1639841 Pubmed8900172 Pubmed9334164 Reactome Database ID Release 43382054 Reactome, http://www.reactome.org ReactomeREACT_16880 Reviewed: Heldin, CH, 2008-11-23 19:29:34 The long splice version of the PDGF-A chain as well as the COOH-terminal part of the PDGF-B precursor contain C-terminal protein motifs that confer retention of the secreted factors. In both the PDGF A- and B-chains, exon 6 encodes a basic sequence that mediates interaction with components of the extracellular matrix. PDGF binds to various types of collagens, thrombospondin and osteopontin; however, the major component of the matrix involved in PDGF binding is likely to be haparan sulphate. The negatively charged sulfate groups on the disaccharide building blocks of heparan sulfate (HS) polysaccharide chains provide binding sites for positively charged amino acid sequence motifs. <br>The precursor of the B-chain may be retained in the matrix; after maturation when the COOH-terminal retention sequence has been cleaved off, the molecule may become more diffusible. has a Stoichiometric coefficient of 2