ubiquitin Tight Junction Complex:TGFB1:TGFBR2:p-TGFBR1:p-PARD6A:RHOA:SMURF1 Tight Junction Complex:TGFB1:TGFBR2:p-TGFBR1:p-PARD6A:Ub-RHOA:SMURF1 SMURF1 ubiquitinates RHOA Authored: Orlic-Milacic, M, 2012-04-04 Edited: Jassal, B, 2012-04-10 Pubmed14657501 Pubmed15761148 Reactome Database ID Release 432160935 Reactome, http://www.reactome.org ReactomeREACT_121146 Reviewed: Huang, Tao, 2012-05-14 SMURF1, recruited to tight junctions through association with phosphorylated PARD6A, ubiquitinates RHOA, leading to RHOA degradation and disassembly of tight junctions (Ozdamar et al. 2005). Disassembly of tight junctions is an important step in epithelial to mesenchymal transition. SMURF1, but not SMURF2, decreases RHOA level, and this effect is proteasome dependent (Wang et al. 2003).