Glc,Fuc-Pre-NOTCH NOTCH NOTCH precursor cleaved to form mature NOTCH Authored: Jassal, B, 2004-12-15 13:08:03 EC Number: 3.4.21 Edited: D'Eustachio, P, 2012-02-06 Edited: Orlic-Milacic, M, 2012-02-10 Pubmed10669757 Pubmed12049766 Pubmed19701457 Pubmed9244302 Pubmed9653148 Pubmed9727485 Reactome Database ID Release 431912369 Reactome, http://www.reactome.org ReactomeREACT_118833 Reviewed: Haw, R, 2012-02-06 Reviewed: Joutel, A, 2004-12-15 The NOTCH receptor is synthesized as a precursor polypeptide (approx. 300 kDa) associated with the endoplasmic reticulum membrane. The mature NOTCH receptor is produced by proteolytic cleavage to form a heterodimer. The enzyme responsible is a furin-like convertase which cleaves the full-length precursor into a transmembrane fragment (NTM) of approximate size 110 kDa and an extracellular fragment (NEC) of approximate size 180 kDa. The mature NOTCH receptor is reassembled as a heterodimer (Blaumueller et al. 1997, Logeat et al. 1998). Both disulfide bonds and calcium-mediated ionic interactions stabilize the heterodimer (Rand et al. 2000, Gordon et al. 2009). This process takes place in the trans-Golgi network . Mammalian NOTCH is predominantly presented as a heterodimer on the cell surface. Although FURIN-mediated cleavage is evolutionarily conserved, it may not be mandatory for Drosophila Notch function (Kidd et al. 2002).