methacrylyl-CoA + H2O <=> beta-hydroxyisobutyryl-CoA EC Number: 4.2.1.17 ISBN0079130356 Reactome Database ID Release 4370870 Reactome, http://www.reactome.org ReactomeREACT_297 The reversible reaction of methacrylyl-CoA and water to form beta-hydroxybutyryl-CoA takes place in the mitochondrial matrix. While crude extracts of human fibroblasts and liver cells have been shown to catalyze the reaction, the specific human enzyme responsible for it has not been identified.