Host genomic DNA IN:viral DNA bound to host genomic DNA with staggered ends viral PIC proteins Target DNA binding Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22 Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22 How the PIC finds favored sites on target DNA has not been fully clarified. Active genes are favored for integration, and favored sequences at the site of integration also influence the reaction. Studies of cells depeleted in PSIP1/LEDGF/p75 suggest that this protein acts as a tethering factor binding HIV PICs near integration target DNA. Access of PICs to sites on chromosomes may be significant, since centromeric alphoid repeats are disfavored for integration, perhaps due to wrapping in compact centromeric heterochromatin. Nucleosomes bound to the integration template also affect target site selection and integration complex binding. Pubmed12202041 Pubmed15314653 Pubmed15677323 Pubmed15802467 Pubmed16175173 Pubmed16260736 Pubmed16291214 Pubmed16311605 Pubmed1760846 Pubmed9557688 Reactome Database ID Release 43175108 Reactome, http://www.reactome.org ReactomeREACT_9054 Reviewed: Bushman, FD, 2006-10-30 22:19:13 IN bound to sticky 3' ends of viral DNA in PIC