Synthesis of phenylacetyl CoA from phenylacetate and Coenzyme A Edited: D'Eustachio, P, 2006-03-23 15:51:01 Phenylacetate and ATP react with coenzyme A to form phenylacetyl CoA, AMP, and pyrophosphate (Vessey et al. 1999). Two human CoA ligases have been characterized that catalyze this reaction efficiently in vitro: acyl-CoA synthetase medium-chain family member 1 (BUCS1) (Fujino et al. 2001) and xenobiotic/medium-chain fatty acid:CoA ligase (Vessey et al. 2003). Their relative contributions to phenylacetate metabolism in vivo are unknown. Pubmed10434065 Pubmed11470804 Pubmed12616642 Reactome Database ID Release 43177157 Reactome, http://www.reactome.org ReactomeREACT_6751 Reviewed: D'Eustachio, P, 2008-05-28 08:30:54 phenylacetate + Coenzyme A + ATP => phenylacetyl-CoA + AMP + pyrophosphate