Acetylcholine Loaded Synaptic Vesicle synaptic vesicle Loading of acetylcholine in synaptic vesicle Acetylcholine is actively transported from the cytosol to the lumen of the clathrin sculpted synaptic vesicle by vesicular acetylcholine transporter. Two protons are exchanged for 1 molecule of acetylcholine. The vesicular acetylcholine transporter is located in the membrane of the clathrin sculpted synaptic vesicle. Authored: Mahajan, SS, 2008-01-14 16:01:52 Pubmed8071310 Reactome Database ID Release 43264615 Reactome, http://www.reactome.org ReactomeREACT_15317 has a Stoichiometric coefficient of 2