DNA-PK:XRCC4:DNA ligase IV:DNA complex associated with ligatable DNA ends ligated DNA double-strand break ends Removal of repair proteins and ligation of the processed ends of the DNA double-strand break Authored: Matthews, L, 2003-09-07 08:47:00 Presumably the proteins must be released from the DNA prior to end joining but precisely when this step occurs is not known. Mg-ATP and the protein kinase activity of DNA-PKcs are required for repair of DNA double strand breaks (Kurimasa et al, 1999; Kienker et al, 2000; Baumann and West, 1998). In vitro, DNA-PK undergoes autophosphorylation which correlates with loss of protein kinase activity and disruption of the DNA-PKcs-Ku-DNA complex (Chan and Lees-Miller, 1996; Douglas et al, 2001; 2002; Merkle et al, 2002). Moreover, autophosphorylation of DNA-PKcs is required for NHEJ in vivo (Chan et al, 2002; Ding et al, 2003), suggesting that autophosphorylation may serve as a mechanism to remove DNA-PKcs from DNA prior to end joining. Pubmed10207111 Pubmed10908332 Pubmed11376007 Pubmed12186630 Pubmed12231622 Pubmed12379113 Pubmed12897153 Pubmed8621537 Pubmed9826654 Reactome Database ID Release 4375928 Reactome, http://www.reactome.org ReactomeREACT_12 has a Stoichiometric coefficient of 2 has a Stoichiometric coefficient of 4