Liganded G12/13-activating GPCRs bind inactive heterotrimeric G-protein G12/13 Liganded G12/13-activating GPCR acts as a GEF for G12/13 The Ligand:GPCR:G12/13 complex dissociates ROCK activation by Rho LARG activation by G alpha 12/13 LARG binds plexin B1 G alpha (12/13) auto-inactivates by hydrolysing GTP to GDP G alpha (13) activates Rho guanine nucleotide exchange factor 1 (p115-RhoGEF) p115-RhoGEF activation of Rac1 Rac1 activation of PI3K GEFs activate RhoA,B,C Inactive G alpha (12/13) reassociates with G beta:gamma PathwayStep5776 PathwayStep5768 PathwayStep5767 PathwayStep5766 PathwayStep5765 PathwayStep5769 PathwayStep5770 PathwayStep5771 PathwayStep5774 PathwayStep5775 PathwayStep5772 PathwayStep5773 G alpha (12/13) signalling events Authored: Jupe, S, 2009-03-18 10:29:41 Edited: Jupe, S, 2009-03-27 13:06:35 Pubmed12588974 Pubmed16102047 Pubmed18814923 Pubmed19212140 Pubmed7559569 Reactome Database ID Release 43416482 Reactome, http://www.reactome.org ReactomeREACT_18407 Reviewed: Akkerman, JW, 2009-06-03 The G12/13 family is probably the least well characterized subtype, partly because G12/13 coupling is difficult to determine when compared with the other subtypes which predominantly rely on assay technologies that measure intracellular calcium. The G12/13 family are best known for their involvement in the processes of cell proliferation and morphology, such as stress fiber and focal adhesion formation. Interactions with Rho guanine nucleotide exchange factors (RhoGEFs) are thought to mediate many of these processes. (Buhl et al.1995, Sugimoto et al. 2003). Activation of Rho or the regulation of events through Rho is often taken as evidence of G12/13 signaling. Receptors that are coupled with G12/13 invariably couple with one or more other G protein subtypes, usually Gq.