Adenylate cyclase converts ATP to 3',5'-cyclic AMP (cAMP) and pyrophosphate Liganded Gi-activating GPCR acts as a GEF for Gi Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi G alpha (i) auto-inactivates by hydrolysing GTP to GDP The Ligand:GPCR:Gi complex dissociates Opsins that act as GEFs for G alpha-t Inactive G alpha (i) reassociates with G beta:gamma PathwayStep3369 PathwayStep5742 PathwayStep5741 PathwayStep5740 PathwayStep5733 PathwayStep5738 PathwayStep5739 PathwayStep5743 G alpha (i) signalling events Authored: Jupe, S, 2009-05-01 15:23:43 Edited: Jupe, S, 2009-05-01 15:23:43 Pubmed3113327 Pubmed9278091 Reactome Database ID Release 43418594 Reactome, http://www.reactome.org ReactomeREACT_19231 Reviewed: Akkerman, JW, 2009-06-03 The classical signalling mechanism for G alpha (i) is inhibition of the cAMP dependent pathway through inhibition of adenylate cyclase. Decreased production of cAMP from ATP results in decreased activity of cAMP-dependent protein kinases. G alpha (i) inhibits adenylate cyclase