RNA Pol II (hypophosphorylated) complex bound to DSIF protein DSIF:NELF:early elongation complex NELF complex NTP Formation of DSIF:NELF:HIV-1 early elongation complex Authored: Matthews, L, Rice, AP, 2005-07-27 00:00:00 Edited: Matthews, L, 2005-07-26 23:29:22 Pubmed10199401 Reactome Database ID Release 43167085 Reactome, http://www.reactome.org ReactomeREACT_6357 This HIV-1 event was inferred from the corresponding human RNA Pol II transcription event. NELF complex is a ~ 300 kDa multiprotein complex composed of 5 peptides (A - E): ~66,61,59,58 and 46 kDa (Yamaguchi et al 1999). All these peptides are required for NELF-mediated inhibition of Pol II elongation. NELF complex has been reported to bind to the pre-formed DSIF:RNA Pol II complex that may act as a scaffold for its binding. NELF-A is suspected to be involved in Wolf-Hirschhorn syndrome. Binding of DSIF:NELF to RNA Pol II CTD results in abortive termination of early elongation steps by the growing transcripts.