AKT binds PDPK1 PIP3 recruits PDPK1 to the membrane TORC2 (mTOR) phosphorylates AKT at S473 PIP3 recruits AKT to the membrane PDPK1 phosphorylates AKT at T308 PathwayStep6507 PathwayStep6506 PathwayStep6505 PathwayStep6504 p-S400-Cot phosphorylates NIK CD28 bound PI3K phosphorylates PIP2 to PIP3 AKT interacts and phosphorylates Cot PI3K binds CD28 CD28 dependent PI3K/Akt signaling Authored: Garapati, P V, 2008-12-16 11:12:19 Edited: Garapati, P V, 2008-12-16 11:12:19 PI3Ks can be activated by a number of different receptors, including the TcR (T cell receptor), co-stimulatory receptors (CD28), cytokine receptors and chemokine receptors. However, the specific roles of PI3Ks downstream of these receptors vary. CD28 contains the YMNM consensus PI3K-binding motif, and PI3K recruitment by CD28 contributes to or complements TCR-dependent PI3K signaling. Activation of PI3K promotes PIP3 production at the plasma membrane and several potential target molecules for this phospholipid have been implicated in PI3K pathways downstream of the TcR and CD28. Of these targets, at least Vav and Akt have been implicated in CD28 costimulation of T cell activation. AKT/PKB connects PI3K to signaling pathways that promote cytokine transcription, survival, cell-cycle entry and growth. Pubmed12670391 Pubmed15046602 Reactome Database ID Release 43389357 Reactome, http://www.reactome.org ReactomeREACT_19358 Reviewed: Bluestone, JA, Esensten, J, 2009-06-01 18:47:33 PathwayStep3871 PathwayStep3872 PathwayStep3870 PathwayStep3874 PathwayStep3873