PathwayStep6367 PathwayStep6366 PathwayStep6369 PathwayStep6368 PathwayStep6373 PathwayStep6371 PathwayStep6372 PathwayStep6370 Arf1 Activation by GBF1 Coat Complex Formation GAP Recruitment to the Coatomer:Arf1-GTP Complex Coatomer:Arf1-GTP:GAP lattice formation on golgi membrane Sculpting and pinching-off of Golgi vessicle Hydrolysis of Arf1-GTP to Arf1-GDP Diffusion of inactive Arf1-GDP from membrane Golgi vesicle lattice disassociation COPI Mediated Transport Authored: Gillespie, ME, 2007-07-23 15:44:13 GENE ONTOLOGYGO:0048205 Pubmed16956762 Reactome Database ID Release 43199997 Reactome, http://www.reactome.org ReactomeREACT_11096 Reviewed: Rush, MG, 2008-01-11 00:00:00 Secretory transport depends on membrane-bounded carriers to move protein and lipid between intracellular compartments. The COPI coat has a central role in this process, creating a sorting domain on the membrane into which cargo proteins, destined to return to the endoplasmic reticulum (ER), concentrate. The membrane domain deforms into a coated bud, pinches off the membrane as a coated carrier, and then uncoats. Successful operation of the COPI coat system is necessary for selective retrieval of protein and lipid components back to the ER.