RTC without viral RNA template RTC with annealed complementary PBS seqments in +sssDNA and -strand DNA Second strand transfer by annealing complementary PBS sequences Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22 Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22 Pubmed10723025 Reactome Database ID Release 43164512 Reactome, http://www.reactome.org ReactomeREACT_9056 Reviewed: Hughes, SH, 2006-10-30 22:00:51 With the removal of all viral genomic RNA and tRNA, the PBS sequence at the 3' end of the plus-strand strong-stop DNA (+sssDNA) is free to pair with the complementary PBS sequence at the 3' end of the minus-strand DNA, to generate a circular structure (Telesnitsky and Goff 1997).