RTC with degraded RNA template and minus sssDNA RTC with nicked minus sssDNA:tRNA primer:RNA template RNase H-mediated degradation of the RNA strand of the -sssDNA:RNA duplex Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22 EC Number: 3.1.26.4 Pubmed10956669 Pubmed11035788 Pubmed1281479 Reactome Database ID Release 43182859 Reactome, http://www.reactome.org ReactomeREACT_9014 Reviewed: Hughes, SH, 2006-10-30 22:00:51 The rate of RNase H cleavage is substantially lower than the rate of DNA synthesis (Kati et al. 1992), so the product of the combined DNA synthesis and RNA degradation events catalyzed by the RT heterodimer mediating minus-strand strong stop DNA (-sssDNA) synthesis is a DNA segment still duplexed with extended viral genomic RNA fragments. In vitro, other RT heterodimers bind the remaining RNA:DNA heteroduplexes and their RNase H domains further degrade the viral genomic RNA (Wisniewski et al. 2000a, b).