GAG polyprotein N-myristoyl GAG polyprotein N-myristoylation of GAG polyprotein by NMT2 Authored: D'Eustachio, P, 2006-07-25 20:21:32 EC Number: 2.3.1.97 Pubmed11527981 Pubmed1548743 Pubmed15613341 Pubmed9506952 Reactome Database ID Release 43184392 Reactome, http://www.reactome.org ReactomeREACT_115774 The amino terminal glycine residue of HIV-1 Gag polyprotein is myristoylated (Henderson et al. 1992). Myristoylation of newly synthesized Gag occurs in the cytosol of the infected host cell, with myristoyl-CoA as the myristate donor and the host cell NMT2 enzyme as the catalyst. Human cells express two isoforms of N-myristoyl transferase (NMT) (Giang and Cravatt 1998). The argumant that the second isoform catalyzes this reaction is indirect, based on the the observations that a stable enzyme:substrate complex forms transiently during the reaction (Farazi et al. 2001), and that Gag polyprotein can be found complexed with NMT2 (but not NMT1) in HIV-1-infected human cells (Hill and Skowronski 2005).