Robo1:Slit2:Glypican-1 Slit2:Glypican-1 Robo1 binds Slit2 Authored: Garapati, P V, 2008-09-05 06:02:05 Edited: Garapati, P V, 2008-09-05 06:02:05 Pubmed10102266 Pubmed10102268 Pubmed12508280 Pubmed17553100 Reactome Database ID Release 43204364 Reactome, http://www.reactome.org ReactomeREACT_19272 Reviewed: Kidd, T, 2009-08-18 The Slit family consists of three members that are all expressed in the ventral midline (floor plate) of the neural tube. Slit 1 is predominantly expressed in the nervous system whereas Slit 2 and 3 are also expressed outside the nervous system.<br>Slit proteins are the ligands for the Robo receptors. In humans there are four robo genes: Robo1, 2, 3 and 4. The extracellular domain of Robo comprises five Ig domains and three Fn domains except for Robo4 (2Ig+2Fn). Ig1 and Ig2 domains of Robo are highly conserved and are important for Slit binding. The concave face of slit's second LRR domain accommodates the Robo's Ig1 and 2 domains. Slit binding with Robo4 is controversial as the interaction is weak and its been observed using the in-vitro methods.<br>