vRNA (Genomic):NP Complex vRNA (genomic) NP binds vRNA Authored: Bortz, E, Garcia-Sastre, A, 2007-02-12 19:39:05 Pubmed15702989 Pubmed17050598 Pubmed17081640 Pubmed17151603 Pubmed9032315 Pubmed9371635 Reactome Database ID Release 43192912 Reactome, http://www.reactome.org ReactomeREACT_9490 Reviewed: Squires, B, 2007-02-12 19:39:22 Viral genomic RNA (vRNA) and complementary RNA (cRNA) are likely bound by the influenza nucleoprotein (NP) immediately upon synthesis. Although two nuclear localization signals have been mapped in the NP, an unconventional N-terminal NLS and a bipartite NLS within amino acids 198-216 (Wang, 1997; Neumann, 1997; Ozawa, 2007), the crystal structure of the NP suggests that only the unconventional NLS is exposed and can be used as a functional NLS (Ye, 2006). This unconvenetional NLS interacts with importins alpha-1 and -2 (Cros et al., 2005; Wang et al., 1997; Buolo et al., 2006). The three-dimensional structure of NP has revealed that NP molecules associate as a trimer, interacting through beta-sheets b5, b6, and b7 in the C-terminal domain of the protein; the viral RNA likely wraps around the outside of the complex (Ye, 2006). has a Stoichiometric coefficient of 3