APOBEC3G:RTC with deaminated minus sssDNA:tRNA primer:RNA template APOBEC3G:RTC with minus sssDNA:tRNA primer:RNA template Deamination of C residues during synthesis of HIV-1 reverse transcript minus-strand Authored: Matthews, L, 2006-06-07 20:09:24 During reverse transcription, APOBEC3G-mediated minus-strand deamination occurs within a CC dinucleotide context over the entire length of the HIV-1 genome (Yu et al., 2004). <br>The polypurine tract is essential for plus strand synthesis and is located at the 3’ end of the retroviral genome. HIV-1 encodes an additional central polypurine tract located in the middle of the genome which also serves as primer for plus strand synthesis.<br>Deamination of the minus strand continues throughout its synthesis with the frequency of deamination events increasing from the 5’ to 3’ regions. A 400bp region downstream of the central polypurine tract seems to be protected from deamination (Wurtzer et al., 2006) EC Number: 3.5.4.13 Edited: Matthews, L, 2007-01-30 11:27:41 Pubmed12808465 Pubmed15098018 Pubmed16537639 Reactome Database ID Release 43180632 Reactome, http://www.reactome.org ReactomeREACT_9435 Reviewed: Mulder, L, 2007-01-30 22:57:00 Reviewed: Simon, V, 2007-01-30 23:07:12