RTC with extending minus strand DNA
RTC with extending second-strand DNA
3' PPT-primed initiation of plus-strand DNA synthesis
Authored: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
EC Number: 2.7.7.7
Edited: Gopinathrao, G, D'Eustachio, P, 2006-05-18 20:10:22
HIV-1 genomic RNA contains a centrally located PPT (cPPT) within the pol gene that, like 3'PPT, is spared by RNase H during minus-strand DNA synthesis and persists to prime plus-strand DNA synthesis. This ribonucleotide primes the synthesis of a plus-strand DNA extending through the U3 and R regions of the HIV sequence and terminating in the PBS region (the tRNA primer-binding site). This DNA segment is known as plus-strand strong-stop DNA (+sssDNA) (Telesnitsky and Goff 1997; Pullen et al. 1993; Huber and Richardson 1990). cPPT priming is important for efficient viral replication (Alizon et al. 1992; Rausch and Le Grice 2004). Several features of cPPT priming in vivo remain to be clarified.
Pubmed10723025
Pubmed15183342
Pubmed1560526
Pubmed1693920
Pubmed7681062
Reactome Database ID Release 43164513
Reactome, http://www.reactome.org
ReactomeREACT_9075
Reviewed: Hughes, SH, 2006-10-30 22:00:51