negatively regulates chromosome segregation negative regulation of cell cycle process regulation of chromosome segregation negative regulation of chromosome segregation down regulation of chromosome segregation down-regulation of chromosome segregation Any process that stops, prevents, or reduces the frequency, rate or extent of chromosome segregation, the process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets. inhibition of chromosome segregation GO:0051985 downregulation of chromosome segregation biological regulation