Abnormal T cell subset distribution Abnormal double-negative T cell proportion 2017-09-03T12:57:22Z Abnormal DN Treg distribution Abnormal Double negative Treg distribution T lymphocytes bearing the T cell receptor (TCR) but lacking CD4, CD8, and markers of natural killer (NK) cell differentiation, i.e., TCR(+)CD3(+)CD4(-)CD8(-), are known as double-negative (DN) T cells and are defined by their capability of inhibiting immune responses via directly killing effector T cells in an antigen specific fashion. Furthermore, DN Treg cells have been shown to develop regulatory activity after encountering specific antigens, partially mediated by the acquisition of MHC-peptide complexes from antigen presenting cells (APCs). Abnormal increase or decrease of double-negative (DN) CD3+CD4-CD8- T cells, measured either as percentage of total CD3+ T cells in the blood, or as absolute number per microliter of blood, compared to a reference range for a given sex and age-group. These are usually measured within the TCR alpha/beta positive population. Abnormal CD4-negative, CD8-negative regulatory T cell distribution Abnormal proportion of double-negative regulatory T cell Abnormal double-negative regulatory T cell distribution