Positive stool infectious agent test Positive stool glutamate dehydrogenase test 2025-08-10T11:09:56Z Detection of C. difficile glutamate dehydrogenase Positive C. difficile glutamate dehydrogenase test Clostridium difficile produces an NAD-specific glutamate dehydrogenase (GDH), which converts l-glutamate into alpha-ketoglutarate through an irreversible reaction. The enzyme GDH is detected in the stool samples of patients with C. difficile-associated disease and serves as one of the diagnostic tools to detect C. difficile infection. Clostridium difficile is the leading cause of hospital-acquired diarrhoea, which ranges in severity from mild diarrhoea to fulminant colitis. Detection of the presence of glutamate dehydrogenase produced by Clostridium difficile in a stool sample.