immortal macrophage cell line cell J-774 HyperCLDB: cl2911 A murine macrophages cell line established from a tumor that arose in a female BALB/c mouse. Its growth is inhibited by dextran sulfate, purified protein derivative, and bacterial lipopolysaccharides. J774 cells have been used for numerous biochemical studies aimed at understanding the physiology of monocytes-macrophages. This cell line synthesizes large amounts of lysozyme and exhibits minor cytolysis but predominantly antibody-dependent phagocytosis. The cells have been shown to express cell-bound receptors for immunoglobulin and complement. J774 cells express high levels of the enzyme nitric oxide synthase and produce large amounts of nitric oxide when activated with IFN-gamma and a low concentration of bacterial lipopolysaccharides (10 ng/mL). Pretreatment of the cells with IL10 inhibits this process in a dose-dependent manner while addition of IL10 at the same time or after IFN-gamma activation is without effect. Treatment of J774 cells with IFN-gamma induces synthesis of IL12 mRNA expression. J774 is used in bioassays to detect M-CSF since its growth is dependent on this factor. The cells constitutively secrete IL1 into the conditioned medium. (http://www.copewithcytokines.de/cope.cgi?key=J774) J774 macrophage cell