has_specified_input PCR assay for detecting B. abortus 16S rRNA YH, YL, ZX a PCR assay for brucellosis that is designed to detect Brucella 16S rRNA. PubMed: 7538508 This is a PCR assay with primers derived from the 16S rRNA sequence of Brucella abortus. The pair of primers chosen amplified a 905bp fragment. As little as 80 fg of Brucella DNA was detected by this method. DNAs from all of the representative strains of the species and biovars of Brucella and from 23 different Brucella isolates were analyzed and yielded exclusively the 905-bp fragment. No amplification was detected with DNAs from 10 strains phylogenetically related to Brucella spp., 5 gram-negative bacteria showing serological cross-reactions with Brucella spp., and 36 different clinical isolates of non-Brucella species. Only Ochrobactrum anthropi biotype D yielded a PCR product of 905 bp, suggesting a closer relationship between Brucella spp. and O. anthropi biotype D. The specificity and high sensitivity of the PCR assay may provide a valuable tool for the diagnosis of brucellosis. B. abortus 16S rRNA PCR forward primer B. abortus 16S rRNA PCR reverse primer PCR assay for brucellosis