has_specified_input has_specified_output achieves_planned_objective omp-2 forward primer omp-2 reverse primer PCR to amplify 193bp region located in Brucella omp-2 gene A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (OMP-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovine, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans. PubMed: 8586678 YH, YL, ZX a PCR assay that has an objective of amplying the 193 bp's region located in Brucella omp-2 gene. objective of amplyfing a 193 region located in gene omp-2 PCR product of PCR assay for detecting Brucella omp-2 polymerase chain reaction