updated AMOS a PCR test for brucellosis that is composed of updated AMOS. YH, YL, ZX PMID: 7650203 Distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. To accomplish this, the PCR-based, species-specific AMOS assay (B. J. Bricker and S. M. Halling, J. Clin. Microbiol. 32:26602666, 1994) was updated to identify Brucella abortus vaccine strains S19 and RB51. Three new oligonucleotide primers were added to the five-primer multiplex Brucella AMOS PCR assay. Identification is based on the number and sizes of six products amplified by PCR. PubMed: 7650203 PCR assay for brucellosis