negatively_regulates macrophage Brucella intracellular replication in macrophage negative regulation of Brucella intracellular replication in macrophage Any process that stops, prevents, or reduces the frequency, rate or extent of the Brucella's l life cycle, the set of processes by which Brucella reproduces and spreads among macrophage cells. reduced intracellular replication in macrophage negative regulation of Brucella intracellular replication