realized in has disposition at some time agent_in_compromised_process Brucella intracellular replication in macrophage artificially mutated Brucella B. abortus purD mutant MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages [Ref6485:Alcantara et al., 2004]. Like the purE mutant, a purD::Tn10 mutant has reduced survival in murine macrophages and reduced virulence in mice [Ref6485:Alcantara et al., 2004]. See. PMID: 15271960 macrophage - Brucella interaction attenuated disposition mouse - Brucella interaction Brucella intracellular survival