realized in has disposition at some time agent_in_compromised_process artificially mutated Brucella macrophage - Brucella interaction attenuated disposition Hela cell - Brucella interaction Brucella intracellular survival Brucella glnD mutant MUTATION: glnD encodes for a uridylyl transferase which is the primary sensor of nitrogen. The glnD mutant via signature-tagged transposon mutagenesis is attenuated in THP1 macrophages and HeLa cells. It supports the hypothesis that the concentration of glutamine in host cells is critical for the intracellular survival of Brucella [Ref6484:Foulongne et al., 2000]. See PMID:10678941