realized in has disposition at some time agent_in_compromised_process Brucella intracellular replication in macrophage artificially mutated Brucella macrophage - Brucella interaction attenuated disposition Brucella intracellular survival B. abortus purL mutant MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages [Ref6485:Alcantara et al., 2004]. Studies with B melitensis purE and B abortus purL mutants, which are purine auxotrophs, and B suis aroC mutants, which can not synthesize aromatic amino acids, indicate that the brucellae also face signicant nutrient limitation during their prolonged residence in host macrophages [Ref6485:Alcantara et al., 2004]. See PMID:15271960