realized in has disposition at some time agent_in_compromised_process artificially mutated Brucella attenuated disposition Hela cell - Brucella interaction Brucella intracellular survival B. abortus aspC mutant MUTATION: aspC encodes for an aminotransferase. B.abortus aspC mutant obtained from randomized miniTn5Km2 transposon mutagenesis showed decreased intracellular survival inside HeLa cells. So B. abortus aspC gene is essential for HeLa cell intracellular growth [Ref6469:Kim et al., 2003]. See PMID:12761078