biological_process biological_process Brucella RicA and Rab2 binding Cell Microbiol. 2011 Jul;13(7):1044-58. doi: 10.1111/j.1462-5822.2011.01601.x. Epub 2011 May 30. Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2. de Barsy M(1), Jamet A, Filopon D, Nicolas C, Laloux G, Rual JF, Muller A, Twizere JC, Nkengfac B, Vandenhaute J, Hill DE, Salcedo SP, Gorvel JP, Letesson JJ, De Bolle X. Author information: (1)URBM, University of Namur (FUNDP), Belgium. Bacteria of the Brucella genus are facultative intracellular class III pathogens. These bacteria are able to control the intracellular trafficking of their vacuole, presumably by the use of yet unknown translocated effectors. To identify such effectors, we used a high-throughput yeast two-hybrid screen to identify interactions between putative human phagosomal proteins and predicted Brucella spp. proteins. We identified a specific interaction between the human small GTPase Rab2 and a Brucella spp. protein named RicA. This interaction was confirmed by GST-pull-down with the GDP-bound form of Rab2. A TEM-β-lactamase-RicA fusion was translocated from Brucella abortus to RAW264.7 macrophages during infection. This translocation was not detectable in a strain deleted for the virB operon, coding for the type IV secretion system. However, RicA secretion in a bacteriological culture was still observed in a ΔvirB mutant. In HeLa cells, a ΔricA mutant recruits less GTP-locked myc-Rab2 on its Brucella-containing vacuoles, compared with the wild-type strain. We observed altered kinetics of intracellular trafficking and faster proliferation of the B. abortusΔricA mutant in HeLa cells, compared with the wild-type control. Altogether, the data reported here suggest RicA as the first reported effector with a proposed function for B. abortus. © 2011 Blackwell Publishing Ltd. PMID: 21501366 [PubMed - indexed for MEDLINE] PMID: 21501366 The biological process that Brucella's RicA protein binding with human Rabs protien. YL de Barsy et al. observed that Brucella protein RicA encoded by gene BMEI0736 from B. abortus interacts with human small GTPase protein Rab2.(bru-interaction paper)