has_part has part has_part has disposition at some time B. abortus virB1 mutant attenuated disposition B. suis 1330 virB1 mutant Information about the mutated molecule: MUTATION: The Brucella abortus virB operon, encoding a type IV secretion system (T4SS), is required for intracellular replication and persistent infection in the mouse model. The products of the first two genes of the virB operon, virB1 and virB2, are predicted to be localized at the bacterial surface. Both mutants were shown to be nonpolar, as demonstrated by their ability to express the downstream gene virB5 during stationary phase of growth in vitro. Both VirB1 and VirB2 were essential for intracellular replication in J774 macrophages. The nonpolar virB1 mutant persisted at wild-type levels, showing that the function of VirB1 is dispensable in the mouse model of persistent infection [Ref6471:Höppner et al., 2005]. A B abortus polar virB1 mutant failed to replicate in HeLa cells, indicating that the virB operon plays a critical role in intracellular multiplication [Ref6471:Höppner et al., 2005]. Polar mutations in the virB1 to virB2 intergenic region or in virB2 reduced the detection of VirB5 to a greater extent than they did that of VirB12. A virB1 mutation also eliminates the transcription of virB12 in B suis [Ref6471:Höppner et al., 2005]. An infection assay with signature-tagged Brucella abortus mutants demonstrated that mutagenesis of the virB1 gene causes attenuation of virulence [Ref6471:Höppner et al., 2005]. NCBIGene: 1164506 PMID: 16272371 The gene virB1 from the strain Brucella suis 1330 is a virulence gene. YH A mutant of strain Brucella suis 1330 that lacks an intact gene virB1. UniProtKB accession: Q9RPY4 BRA0069 type IV secretion system protein VirB1