has_part has part has_part has disposition at some time attenuated disposition B.suis norD mutant B. suis 1330 norD mutant PMID: 16495577 NCBIGene: 1164688 A mutant of strain Brucella suis 1330 that lacks an intact gene norD. UniProtKB accession: Q8FX38 The gene norD from the strain Brucella suis 1330 is a virulence gene. YH Information about the mutated molecule: MUTATION: A mutant of Brucella suis bearing a Tn5 insertion in norD , the last gene of the operon norEFCBQD, encoding nitric oxide reductase, was unable to survive under anaerobic denitrifying conditions (more-than-5log reduction in viable counts). As a consequence of the norD mutation , NO might not be further reduced to N2O by the NO reductase and it could become toxic for the bacteria. The infection of resting macrophages showed that the norD mutant and the wild-type strain displayed similar rates of multiplication. On the contrary, activation of J774A.1 cells by LPS and IFN was accompanied by a more-thantenfold attenuation of the norD mutant at 48 h p. i. [Ref6475:Loisel-Meyer et al., 2006]. norD norD protein