has_part has part has_part has disposition at some time attenuated disposition Brucella recA mutant B. suis 1330 recA mutant The gene recA from the strain Brucella suis 1330 is a virulence gene. NCBIGene: 1166878 A mutant of strain Brucella suis 1330 that lacks an intact gene recA. YH PMID: 12414170 UniProtKB accession: P65976 Information about the mutated molecule: FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage (By similarity)(Swiss-Prot: P65976). SUBCELLULAR LOCATION: Cytoplasm (By similarity)(Swiss-Prot: P65976). SIMILARITY: Belongs to the recA family(Swiss-Prot: P65976). MUTATION: The RecA mutant was more sensitive than the parental strain to killing by MMS. When administered intraperitoneally to BALBc mice, numbers of bacteria per spleen were consistently lower in animals infected with the RecA mutant than with the parental strain. However, both the RecA mutant and parental strain persisted in mice through 100 days post-infection. These results indicate that RecA is not crucial for persistence of B abortus in mice [Ref6497:Halling, 2002]. The B abortus RecA mutant was virulent in mice, but its course of infection in mice differed from that of the parental strain. The infectious cycle of the parental strain in the mouse model was biphasic. During the rst week, there was an initial rise in cfu of B abortus 2308 in the spleen followed by a decrease during the second week. This phase was followed by a second phase in which B abortus S2308 persisted and slowly increased in numbers in the spleen . Though fewer RecA mutants were found in the spleens of mice infected intraperitoneally in the early stages of the infection and no large initial rise was seen, the same numbers were found as the parental strain 100 days post -infection. This suggests that collectively, different loci are involved to varying extents in the initial infection and the persistence phase [Ref6497:Halling, 2002]. recA recombinase A